Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species

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Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species.

Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 s...

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Monoclonal Antibodies

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Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays: Considerations for Aquatic Toxicologists

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ژورنال

عنوان ژورنال: Diseases of Aquatic Organisms

سال: 2010

ISSN: 0177-5103,1616-1580

DOI: 10.3354/dao02268